High-Performance Liquid Chromatographic Measurement of Cyclophosphamide in Serum

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Abstract
The analysis of cyclophosphamide [N,N-bis(2-chlorethyl)-tetrahydro-2H-1,3,2-oxazaphosphorin-2-amine-2-oxide] by high performance liquid chromatography, using UV detection, is described. The method will enable measurement of serum concentrations of cyclophosphamide over a period of .apprx. 24 h after a dose of 150 mg; it requires 1 ml of serum. The between-day precision of the assay, at concentrations of 0.3, 1.0 and 15.0 mg/l, generated coefficients of variation of 11.8, 12.2 and 7.7%, respectively. Percentages of analytical recovery of cyclophosphamide and internal standard (5-ethyl-5-p-tolylbarbituric acid) were 63 and 73%, respectively. Preliminary data providing the half-life for 2 patients with normal renal function are presented.