ELECTROPHORETIC PATTERN OF GAMMA-GLUTAMYL TRANSFERASE IN SERUM AND ITS ALTERATION BY CHYLOMICRONS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 14  (12) , 589-594
Abstract
Using the sera from 20 patients with elevated .gamma.-glutamyl transferase activity (EC 2.3.2.2) due to intra- or posthepatic cholestasis, the enzyme was separated into 4 bands by cellulose acetate foil electrophoresis. The .gamma.-glutamyl transferase pattern permitted no differentiation between intra- or posthepatic cholestasis. Protein and lipid electrophoresis was performed simultaneously for each serum. A .gamma.-glutamyl transferase band, which appeared at the origin in 11 sera, was only observed in lipemic sera containing chylomicrons. This band of .gamma.-glutamyl transferase does not represent a true isoenzyme, because it results from a complex between chylomicrons and .gamma.-glutamyl transferase, which is separated with the chylomicrons, or is produced by the mixing of sera.