An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis
- 11 July 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (13) , 3813-3821
- https://doi.org/10.1093/nar/18.13.3813
Abstract
A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-0-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.Keywords
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