SEROLOGIC ACTIVITIES AND SPECIFICITIES OF ELEVEN HUMAN LEUKOCYTE ANTISERA PRODUCED BY PLANNED IMMUNIZATION

Publisher Website
Google Scholar
Abstract
Eleven leukocyte antisera, produced in humans by immunization with skin grafts and/or leukocyte injections, and selected for initial activity with the cytotoxic assay, were for comparison examined for agglutinating activity on a random leukocyte panel. The frequency of positive reactions in the cytotoxic assay tended to exceed that in the agglutination assay for 7 of the 11 sera. Two of the sera reacted with significantly greater frequency by cytotoxicity. One serum, apparently monospecific, reacted identically with a panel of 46 leukocyte donors. Six of the 11 antisera showed a significant association in the two assays when tested on the panel simultaneously. The specificities of the agglutinins in the sera were determined by using a leukocyte panel which had previously been phenotyped for antigens of Group 4 and the LA system. Seven sera appeared to contain antibodies for at least one of the following antigens: LAI, LA2, 4a, 4b, and 4d. The tests for specificity by the cytotoxic assay supported these findings. One serum had previously been shown to detect the antigen 7d. The association analysis indicated that three of the sera may be identifying new antigens.

This publication has 0 references indexed in Scilit: