Juvenile Hormone III as a Natural Ligand for Photoaffinity Labelling of JH-Binding Proteins
Open Access
- 1 December 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 39 (11-12) , 1145-1149
- https://doi.org/10.1515/znc-1984-11-1226
Abstract
Juvenile hormone III, i.e. methyl 10,11-epoxy-3,7-dimethyl-2E,6E-dodecadienoate, was used for photoaffinity labelling of JH-binding proteins. Irradiation of haemolymph from Manduca sexta and Locusta migratoria in the presence of racemic [10-3H]JH-III results in covalent incorporation of up to 3.2% of the radioactivity into an acetone insoluble protein fraction. Resolution of the proteins by HPLC and by SDS-PAGE confirms the presence of radiolabelled JH-binding proteins. Proteins not involved in JH-binding in insect haemolymph are labelled photochemically with lower yields only. Thus, JH-III is a suitable natural ligand for photoaffinity labelling of high-affinity binding sites. Use of the natural hormone instead of a hormone analog bears the major advantage that the binding with a JH-binding protein will occur at specific sites.Keywords
This publication has 0 references indexed in Scilit: