Evaluation of membrane adsorption-epifluorescence microscopy for the enumeration of bacteria in coastal surface films
- 1 December 1981
- journal article
- research article
- Published by Springer Nature in Microbial Ecology
- Vol. 7 (4) , 365-369
- https://doi.org/10.1007/bf02341431
Abstract
We have evaluated a method for enumerating surface slick bacteria by combining a membrane adsorption procedure with epifluorescence microscopy. Various chemicals were investigated for their ability to enhance bacterial elution from the membrane filters. The results of the elution-epifluorescence method were compared to plate counts and to direct epifluorescence counts of the sampling membrane filters. In all tests, the elution-epifluorescence technique yielded significantly higher bacterial concentrations.This publication has 15 references indexed in Scilit:
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