An autoanalyzer test to determine immunoglobulin class and IgG subclass of blood group antibodies

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Abstract
An automated antiglobulin test was used to characterize the Ig class and IgG subclass of human red blood cell-bound antibodies. Ig classes and IgG subclasses of some clinically significant antibodies (anti-D and anti-CD, anti-Fy, anti-Jka) as well as of clinically insignificant anti-Chido antibodies were determined. Eighteen anti-Duffy antibodies were structurally homogeneous and were mostly composed only of IgG1. Of the 16 anti-Fya antibodies studied only 3 had IgG2, and 4 had IgM molecules. Ten anti-Jka antibodies were heterogeneous, but all samples were either IgG1 or IgG3, or both. The most prominent Ig of 12 hyperimmune anti-Rh (anti-D and anti-CD) antibodies was IgG1. Under test conditions IgG3 and very low concentrations of IgG2 and IgG4 molecules were also detected in the anti-Rh antibodies.