Abstract
Summary: The growth of and interactions between the glasshouse pathogens,Rhizoctonia solaniKühn,Fusarium oxysporumSchlect.,Pyrenochaeta lycopersiciSchneider & Gerlach,Phomopsis sclerotioidesvan Kesteren,Sclerotinia sclerotiorum(Lib.) de Bary andBotrytis cinereaPers. and the antagonistic fungi,Trichoderma harzianumRifai,Trichoderma viridePers.,Coniothyrium minitansCampbell,Gliocladium roseum(Link.) Bain andPythium oligandrumDreschler were examined on tap water agar, soil extract agar and potato dextrose agar. The medium had a significant effect on the growth rates of the fungi as well as the production of, and response to, volatile and non‐volatile antibiotic compounds and hyphal interactions. Responses to the media appeared to be related both to inherent properties of the fungi and to their natural ecological behaviour. The significance ofin vitroassays in screening systems for biological control agents of soil‐borne plant pathogens is discussed.

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