N‐terminal‐methionylated interleukin‐1β has reduced receptor‐binding affinity
- 4 May 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 215 (1) , 160-164
- https://doi.org/10.1016/0014-5793(87)80133-3
Abstract
The receptor-binding affinity of recombinant-derived interleukin-1β containing unprocessed N-terminal methionine (MAPV-) was 10-fold lower than protein containing the authentic N-terminal sequence (APV-). Structural analysis of the methionylated and non-methionylated proteins by NMR spectroscopy detected no (or minor) conformational differences. The differences in binding affinity, therefore, suggest that the additional N-terminal methionine causes a small, direct or indirect, perturbation of the receptor-binding region.Keywords
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