RNA-dependent RNA polymerase of tobacco plants
- 1 May 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (5) , 2122-2124
- https://doi.org/10.1073/pnas.75.5.2122
Abstract
Several properties of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) active fractions obtained from tobacco plants, be they uninfected, infected with tobacco mosaic virus, or infected with tobacco necrosis virus, were compared. By the seven criteria tested, the RNA-dependent RNA polymerase from these three sources behaved the same, although its activity is greatly but variably stimulated by the two virus infections. It thus appears probable that these two viruses do not code for this enzyme, but rely for their replication on their ability to stimulate production of a host enzyme. The conclusion that cells contain RNA replicating capability represents a modification of the central dogma of molecular biology.Keywords
This publication has 13 references indexed in Scilit:
- RNA-dependent RNA polymerases in uninfected and in alfalfa mosaic virus-infected tobacco plantsVirology, 1977
- RNA polymerase from tobacco necrosis virus-infected and uninfected tobaccoVirology, 1977
- Host and virus specific RNA polymerases in alfalfa mosaic virus infected tobacco.1976
- Rapid, multisample isoelectric focusing in sucrose density gradients using conventional polyacrylamide electrophoresis equipment: A two-peak transient in the approach-to-equilibriumAnalytical Biochemistry, 1975
- Cucumber mosaic virus-induced RNA polymerase: Partial purification and properties of the template-free enzymeVirology, 1974
- RNA-dependent RNA synthesis in rat brainNature, 1974
- Reticulocyte RNA-Dependent RNA PolymeraseProceedings of the National Academy of Sciences, 1973
- In vitro synthesis of double-stranded RNA by an enzyme system isolated from tobacco leavesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Possible identification of a protein in brome mosaic virus infected barley as a component of viral RNA polymeraseVirology, 1973
- Characterization and specificity of soluble RNA polymerase of brome mosaic virusVirology, 1973