Further studies on a particulate enzyme preparation from nodules of soybean plants

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Abstract
Further investigations have been conducted on the properties of a particulate enzyme complex isolated from soybean-nodule bacteriods. It has been shown that the system not only will catalyze the reduction of nitrate to nitrite by use of DPNH or succinate as electron donors, but also will catalyze the transfer of electrons from succinate to O2. This process is inhibited by nitrate, suggesting that both O2 and nitrate compete for electrons from succinate to oxyhemo-globin isolated from nodules. Efforts to demonstrate a succinate-dependent reduction of nodule hemiglobin to hemiglobin in the presence of the enzyme complex were negative. The role of nodule hemoglobin in nodule respiration and N fixation has been discussed briefly.