NEUTRALIZING ANTIBODY TO POLIOVIRUSES IN NORMAL HUMAN URINE*

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Abstract
Neutralizing activity for types 1 and 3 poliovirus was demonstrated in protein concentrates prepared from the urine of a number of normal subjects. The biologic characteristics of the neutralizing activity in the urine concentrates resembled those of specific antibody found in serum. Neutralizing antibody in the urine was usually associated with high titers of the homologous antibody in the serum; it was not demonstrable in the absence of homotypic antibody in serum obtained at the same time, but it was not demonstrated in urine from some subjects who had high serum titers. In some of the subjects the urinary antibody was first demonstrated 1 week or more after a booster dose of poliovirus vaccine, along with the appearance or increase in titer of the homotypic antibody in the serum. The plaque-reduction method combined with immuno-inactivation permitted the demonstration of poliomyelitis -neutralizing antibody in small amounts which could not be demonstrated by the conventional tube assay, even when preceded by immuno-inactivation.

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