Identification and Differentiation of Legionella pneumophila and Legionella spp. with Real-Time PCR Targeting the 16S rRNA Gene and Species Identification by mip Sequencing
- 1 September 2006
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (9) , 6394-6398
- https://doi.org/10.1128/aem.02839-05
Abstract
Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non- L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.Keywords
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