Reverse pattern in differential Giemsa staining of sister chromatids.
- 1 January 1978
- journal article
- research article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 53 (2) , 111-116
- https://doi.org/10.1266/jjg.53.111
Abstract
Chinese hamster strain cells cultured with 20 .mu.M BrdU [5-bromodeoxyuridine] for 2 or 3 rounds of replication were routinely air-dried. The chromosome preparations were treated with 0.001% trypsin at room temperature for 5 to 10 s, or incubated in 0.1 M Na2HPO4 at 37.degree. C for 2 to 4 h. These preparations were then stained with alkaline Giemsa solution (1.5% in 0.1 M Na2HPO4) for 5 to 10 min. These procedures resulted in a reverse type of sister chromatid differential staining; darkly stained BB-chromatids and faintly stained TB-chromatids.This publication has 4 references indexed in Scilit:
- “Reverse” differential staining of sister chromatidsExperimental Cell Research, 1977
- Differential giemsa staining of sister chromatids after extraction with acidsChromosoma, 1977
- Influence of bromodeoxyuridine on the stability and function of polytene chromosomesChromosoma, 1977
- Mechanism of differential staining of BUdR-substituted Vicia faba chromosomesExperimental Cell Research, 1976