SERUM REGULATION OF THE ESTROGEN RESPONSIVENESS OF THE HUMAN-BREAST CANCER CELL-LINE MCF-7

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (3) , 1244-1250
Abstract
Initial studies on a shipment of MCF-7 cells, failed to confirm their estrogen responsiveness. Significant increases in thymidine incorporation, thymidine kinase activity and in cell numbers were observed in response to 10-8 M estradiol. Subsequently the cells failed to show a response to estradiol. A growth response to estradiol could be restored by increasing the serum concentration in the medium. Cells grown in 15% serum (calf or human) responded to estradiol with increased rates of growth and thymidine incorporation and increased activities of thymidine kinase and DNA polymerase. There may be present in serum a factor which can influence the expression of a growth response to estradiol.