Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure
Open Access
- 23 June 2003
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 161 (6) , 1035-1040
- https://doi.org/10.1083/jcb.200301099
Abstract
Very little is known about how cellular osmosensors monitor changes in osmolarity of the environment. Here, we report that in yeast, Sln1 osmosensor histidine kinase monitors changes in turgor pressures. Reductions in turgor caused by either hyperosmotic stress, nystatin, or removal of cell wall activate MAPK Hog1 specifically through the SLN1 branch, but not through the SHO1 branch of the high osmolarity glycerol pathway. The integrity of the periplasmic region of Sln1 was essential for its sensor function. We found that activity of the plant histidine kinase cytokinin response 1 (Cre1) is also regulated by changes in turgor pressure, in a manner identical to that of Sln1, in the presence of cytokinin. We propose that Sln1 and Cre1 are turgor sensors, and that similar turgor-sensing mechanisms might regulate hyperosmotic stress responses both in yeast and plants.Keywords
This publication has 33 references indexed in Scilit:
- How do the polyene macrolide antibiotics affect the cellular membrane properties?Published by Elsevier ,2003
- Osmotic Stress Signaling and Osmoadaptation in YeastsMicrobiology and Molecular Biology Reviews, 2002
- The Arabidopsis AHK4 Histidine Kinase is a Cytokinin-Binding Receptor that Transduces Cytokinin Signals Across the MembranePlant and Cell Physiology, 2001
- Identification of CRE1 as a cytokinin receptor from ArabidopsisNature, 2001
- A Transmembrane Hybrid-Type Histidine Kinase in Arabidopsis Functions as an OsmosensorPlant Cell, 1999
- Yeast HOG1 MAP Kinase Cascade Is Regulated by a Multistep Phosphorelay Mechanism in the SLN1–YPD1–SSK1 “Two-Component” OsmosensorCell, 1996
- From acids to osmZ: multiple factors influence synthesis of the OmpF and OmpC porins in Escherichia coliMolecular Microbiology, 1996
- Activation of Yeast PBS2 MAPKK by MAPKKKs or by Binding of an SH3-Containing OsmosensorScience, 1995
- A two-component system that regulates an osmosensing MAP kinase cascade in yeastNature, 1994
- An Osmosensing Signal Transduction Pathway in YeastScience, 1993