Fura 2 analysis of cytosolic calcium regulation in elutriated rat gastric parietal cells

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Abstract
The calcium probe, Fura 2, is used to establish and partially characterize histamine‐, carbachol‐ and forskolin‐induced calcium transients in enriched parietal cell populations prepared by centrifugal elutriation of dispersed rat fundic mucosa cell isolates. The magnitude of the maximal carbachol response, which is blocked by atropine but not cimetidine, is nearly five times that of histamine or forskolin. Time to peak responses for carbachol, forskolin, and histamine are approximately 7, 17, and 28 sec, respectively. Carbachol‐, histamine‐, and forskolin‐induced increases in Fura 2 fluorescence appear dependent upon extracellular calcium, since these responses are attentuated in low calcium media and blocked by EGTA in low‐calcium media or by lanthanum in high‐ or low‐calcium medium. Trifluoperazine and fenoctimine, at concentrations that inhibit secretion, have no effect on either carbachol‐ or histamine‐induced increases in cytosolic calcium. Seven major calcium/EGTA‐sensitive phosphopro‐teins are identified by SDS‐PAGE electrophoresis of ATP 32P‐labeled cell sonicates. We conclude that cytosolic calcium in enriched rat gastric parietal cell populations is regulated by secretagogue receptor‐controlled calcium channels. We postulate that these channels may be controlled by cyclic AMP‐dependent phosphorylation, since neither changes in cyclic AMP nor calcium alone mediate the effects of secretagogues entirely, but the interplay between these two second‐messenger systems potentiates the actions of these agents. The role of cytosolic calcium as a second messenger in secretagogue action appears similar to that of cyclic AMP in that a specific cellular concentration must be reached to initiate acid secretion.

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