A simple method for the isolation of intact mesophyll protoplasts from cereal plants
- 1 December 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 18 (6) , 1357-1362
- https://doi.org/10.1093/oxfordjournals.pcp.a075558
Abstract
Intact mesophyll protoplasts from cereal plants were easily prepared by incubating leaves with the abaxial epidermis peeled off at 20–25°C for 2–3 hr in 0.6 M mannitol containing 1% cellulase at pH 5.6. From one gram (fresh weight) of leaves 1.5–6×106 protoplasts, more than 90% of which were morphologically intact, could be obtained. Protoplasts isolated from wheat, oat, corn and barley were efficiently infected with brome mosaic virus (BMV), and supported viral multiplication.This publication has 0 references indexed in Scilit: