Purification and Characterization of Alanine Carrier Isolated from H-Proteins of Bacillus subtilis
- 1 February 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 83 (1) , 307-311
- https://doi.org/10.1111/j.1432-1033.1978.tb12095.x
Abstract
Alanine transport carrier was isolated and purified from H‐proteins of Bacillus subtilis. The purified carrier preparation was homogeneous in migration on polyacrylamide gels containing urea or sodium dodecyl sulfate. Electrophoresis on polyacrylamide gels containing dodecyl sulfate showed a single band of molecular weight of about 7500. 1 mol alanine was bound/mol carrier protein with a dissociation constant of 0.2 μM. The binding was inhibited by p‐chloromercuribenzoate and the inhibition was reversed by dithiothreitol.This publication has 10 references indexed in Scilit:
- Isolation and Characterization of Hydrophobic Proteins (H Proteins) in the Membrane Fraction of Bacillus subtilisEuropean Journal of Biochemistry, 1976
- Energy Coupling in Membrane Vesicles of Escherichia coliPublished by Elsevier ,1974
- Effect of glucose on the biosynthesis of the membranes of bacillusBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Solubilization and Partial Purification of Amino Acid-Specific Components of the D-Lactate Dehyrogenase-Coupled Amino Acid-Transport SystemsProceedings of the National Academy of Sciences, 1972
- Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresisArchives of Biochemistry and Biophysics, 1968
- An apparatus for preparative temperature-regulated polyacrylamide gel electrophoresisAnalytical Biochemistry, 1964
- Measurement of protein-binding phenomena by gel filtrationBiochimica et Biophysica Acta, 1962
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949