AN ARGYROPHIL-III METHOD FOR THE DEMONSTRATION OF SMOOTH-MUSCLE CELLS IN LIGHT AND POLARIZATION MICROSCOPY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 84  (2) , 139-142
Abstract
An esterification with n-butyl alcohol containing 0.5% sulfuric acid (at 56.degree. C, for 16 h) followed by a treatment in a special physical developer renders smooth muscle cells [of animals] as well as other contractile elements (striated muscle, cilia, flagella, myoepithelial cells) visible in light microscope and birefringement in polarization microscope. A few kinds of non-contractile tissue components without oriented fibrillary structure also stain but do not display birefringence.

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