Sulfur-deficient transfer ribonucleic acid in a cysteine-requiring, "relaxed" mutant of Escherichia coli.
- 1 December 1969
- journal article
- Vol. 100 (3) , 1322-7
Abstract
A cysteine-requiring mutant of the parent strain Escherichia coli Hfr Cavalli (RC(rel), Met(-), lambda) has been isolated. The mutant was selected by using replica plating after mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine. The mutation appears to be in the gene for sulfite reductase, since the mutant could utilize sulfide but not sulfite as a sulfur source. The mutant was found to be RC(rel) with respect to both methionine and cysteine. During cysteine starvation, transfer ribonucleic acid (tRNA) deficient in 4-thiouracil was produced, and in vivo studies indicate that this tRNA can accept sulfur groups to a greater extent than normal tRNA. Further, there were differences both in the rate and extent of amino acid acceptance between normal and sulfur-deficient tRNA. This suggests that thionucleotides are involved in at least one of the biological functions of the tRNA molecule.This publication has 16 references indexed in Scilit:
- Differential aminoacylation of three species of isoleucine transfer RNA from Escherichia coliJournal of Molecular Biology, 1969
- Role of modifications in tyrosine transfer RNA: A modified base affecting ribosome bindingJournal of Molecular Biology, 1969
- Thionucleotides in transfer ribonucleic acid. Addition of N-ethylmaleimide and formation of mixed disulfides with thiol compoundsBiochemistry, 1968
- Thiobases in Escherichia coli Transfer RNA: 2-Thiocytosine and 5-Methylaminomethyl-2-thiouracilScience, 1968
- Cytokinin from Soluble RNA of Escherichia coli : 6-(3-Methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurineScience, 1968
- RNA thiolase: the enzymatic transfer of sulfur from cysteine to sRNA in Escherichia coli extracts.Proceedings of the National Academy of Sciences, 1966
- Ribonucleic Acid Sulfur-Transferase Activity in Extracts from Escherichia coliCold Spring Harbor Symposia on Quantitative Biology, 1966
- A reversible oxidative in activation of specific transfer RNA species.Proceedings of the National Academy of Sciences, 1965
- The Nature of the RNA Synthesized During Conditions of Unbalanced Growth in E. coli K12W-6*Biochemistry, 1963
- A GENETIC LOCUS FOR THE REGULATION OF RIBONUCLEIC ACID SYNTHESISProceedings of the National Academy of Sciences, 1961