Observations on the Nature of a Genetic Cellular Resistance to Avian Tumor Viruses2

Publisher Website
Google Scholar
Abstract
The subgroup A pseudotype of the Bryan high-titer strain of Rous sarcoma virus [RSV (RAV-1)] and chick embryo fibroblasts resistant to subgroup A (C/A and C/AB) were chosen for study. Attempts to infect resistant cells with free virus showed that resistance was very efficient, if not absolute. Resistant cells removed virus from the medium as efficiently as susceptible cells, which indicated that genetic cellular resistance did not affect adsorption. However, both C/A and C/AB cells synthesized RSV (RAV-1) after growth in contact with lethally irradiated RSV (RAV-1)-producing susceptible cells. Therefore, the genetically controlled block apparently occurred at the level of penetration or uncoating, but did not affect the cell's ability to synthesize the excluded virus, provided the viral genome had entered. The mechanism of penetration of the viral genome into resistant cells during cocultivation with irradiated virus-producing cells is not clear. Possible mechanisms are discussed.

This publication has 0 references indexed in Scilit: