Automatic control of dissolved oxygen levels in fermentations

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Abstract
Dissolved oxygen levels in fermentation systems are affected by (a) gas flow rate, (b) degree of agitation, and (c) oxygen partial pressure in the gas. Since the first two variables cannot easily be separated, a method for dissolved oxygen control has been devised based on the variation of oxygen partial pressure in the gas. Oxygen and nitrogen are fed to a special mixing valve capable of varying the proportions of the two gases while keeping the total flow rate constant. A dissolved oxygen electrode and control system adjust the valve setting to automatically maintain a preset oxygen level. Oxygen analyzers, monitoring the inlet and outlet gas streams, provide additional information from which oxygen absorption and uptake rates may be determined. The system provides a means for the quantitative and independent study of aeration and agitation effects. In addition, it offers a basis for routine control of oxygen levels in plant fermentation equipment.

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