Effect of FSH Treatment on LH and FSH Receptors in Chronic Cystic-Ovarian-Diseased Dairy Cows2

Publisher Website
Google Scholar
Abstract
This experiment was conducted to 1) determine whether chronic cystic-ovarian-diseased (CCOD) cows fail to respond to luteinizing hormone (LH) treatment because of a lack of adequate ovarian LH receptors and 2) determine the effect of follicle stimulating hormone (FSH) treatment on ovarian LH and FSH receptors in ovaries of CCOD cows. The CCOD cows were those that did not resume cyclic ovarian activity after repeated treatment with human chorionic gonadotropin (hCG) and(or) LH-releasing hormone (LHRH) and were considered chronic by veterinarians. Thirteen CCOD cows were purchased from producers; six of them were injected with 5 mg FSH twice daily for 3 or 5 d (TCCOD) and the remaining seven remained untreated. Seven control (noncystic) cows in the luteal phase of the estrous cycle were injected with Lutalyse approximately 48 to 50 h before slaughter so they would be in the follicular phase (FP) of the cycle at the time of slaughter. Analysis of serum and pituitaries showed no differences (P>.05) in mean concentrations of serum or pituitary LH and FSH or pituitary LHRH receptor concentration and affinity among FP, CCOD and TCCOD cows. Ovarian follicle wall concentrations of receptors for LH (3.2 ± .6; 13.0 ± 2.5; 22.4 ± 5.1 fmol/mg protein) and FSH (10 ± 2.6; 43 ± 7.2; 29 ± 6.7 fmol/mg protein) were lower (P<.05) in CCOD cows compared with FP and TCCOD cows, respectively. The same pattern was observed for concentrations of granulosa cell LH and FSH receptors. The follicular fluid estrogen-to-androgen (testosterone and androstenedione; E:A) ratio was >1 in seven of seven FP, 1 in five of six TCCOD cows. Ovaries of CCOD cows lack LH and FSH receptors when compared with FP cows, and these receptor concentrations can be increased to normal levels by treatment with exogenous FSH. Copyright © 1986. American Society of Animal Science . Copyright 1986 by American Society of Animal Science.

This publication has 0 references indexed in Scilit: