Micro-Scale Frontal Affinity Chromatography with Mass Spectrometric Detection: A New Method for the Screening of Compound Libraries
- 31 December 1998
- journal article
- research article
- Published by Wiley in Angewandte Chemie International Edition in English
- Vol. 37 (24) , 3383-3387
- https://doi.org/10.1002/(sici)1521-3773(19981231)37:24<3383::aid-anie3383>3.0.co;2-c
Abstract
In one run the binding constants Kd for all the active components of a ligand library at sub-microgram quantities can be determined. A mixture of ligands is continuously infused through a column of immobilized receptor, and the eluent analyzed by electrospray mass spectrometry. From the affinity chromatogram produced (see picture) the breakthrough volume of a single compound and hence its Kd value can be determined.This publication has 0 references indexed in Scilit: