Effect of oxygen-transfer rate on production of L-asparaginase by Erwinia aroideae

Publisher Website
Google Scholar
Abstract
A variety of aeration and agitation conditions were used to investigate the oxygen demand of Erwinia aroideae for growth and L-asparaginase production in a 14-liter fermenter. Maximum L-asparaginase activity of 4.25 IU/ml was obtained when the fermentation medium was aerated at 0.5 volumes air per volume medium per minute and agitated at 500 rpm. However, the specific L-asparaginase activity was not affected significantly by the rate of oxygen transfer. In most instances the level of dissolved oxygen decreased to zero before harvesting. KLa rather than dissolved oxygen as measured during the fermentation cycle is a more satisfactory index to determine the oxygen requirement for cell growth and asparaginase production. The optimum level of KLa for L-asparaginase production was 0.98 min−1. However, a higher value of KLa of 1.2–1.9 min−1 was needed to achieve the highest growth.

This publication has 0 references indexed in Scilit: