The Culture of Large Free-Living Amebae

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Abstract
A method of culture of A. dubia Schaeffer and A. proteus Pallas (Leidy) is described. Technique for the establishment of laboratory cultures from material brought in from the field; various methods of establishing sub-cultures in Syracuse watch-glasses and in finger bowls are described in detail. The precise quantities of nutrient material, viz., timothy hay, oat and wheat grains, to be used with a definite volume of water are given. A discussion of the conditions affecting the growth of amebae in mass cultures considers the relative value of different kinds of water. Cultures were grown at room temp. (14.5-24[degree] C). The pH of water used was 6.4-7.2; that of thriving cultures, 6.8-7.1. Cultures grow almost equally well in the presence or absence of green algae. No particular need for extra aeration other than that which occurs in the regular changes of medium is indicated. A complete list of organisms with which the amebae have been grown is given[long dash]those most valuable as food and for other considerations are indicated. The weak concentration of the culture medium and the lack of a "prepared" medium are fundamental features of the method described. No "depression" periods nor formation of cysts have been observed.

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