Translation of Encephalomyocarditis Virus RNA in vitro Yields an Active Proteolytic Processing Enzyme
- 1 April 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 85 (2) , 457-462
- https://doi.org/10.1111/j.1432-1033.1978.tb12260.x
Abstract
Unlike other cell-free translation systems, the mRNA-dependent reticulocyte lysate can translate encephalomyocarditis virus RNA efficiently and completely when supplemented with heterologous tRNA. Cleavage of the nascent polypeptide chain occurs, and 1 of the translation products appears to be a specific proteolytic enzyme which correctly processes the primary products. The identity of the proteins made in vitro was verified by comparison with infected [murine myeloma MOPC 21] cell proteins on dodecylsulfate/polyacrylamide gels and by mapping their coding sequences on the viral genome.This publication has 40 references indexed in Scilit:
- The Role of Complexes between the 40-S Ribosomal Subunit and Met-tRNAMetf in the Initiation of Protein Synthesis in the Wheat-Germ SystemEuropean Journal of Biochemistry, 1977
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- The Synthesis of Encephalomyocarditis Virus Polypeptides in Infected L-Cells and Cell-Free SystemsEuropean Journal of Biochemistry, 1974
- The Nucleotide Sequence of the Cytoplasmic Initiator Transfer RNA of a Mouse Myeloma CellEuropean Journal of Biochemistry, 1974
- The Initiation of Protein Synthesis Directed by the RNA from Encephalomyocarditis VirusEuropean Journal of Biochemistry, 1973
- Protein synthesis directed by encephalomyocarditis virus RNA II. The in vitro synthesis of high molecular weight proteins and elements of the viral capsidBiochemical and Biophysical Research Communications, 1971
- Effect of temperature on the cleavage of polypeptides during growth of LSc poliovirusJournal of Molecular Biology, 1971
- The Synthesis of Protein by Mammalian RNA VirusesPublished by Wiley ,1971
- Structure and function of mammalian ribosomes: II. Exchange of ribosomal subunits at various stages of in vitro polypeptide synthesisJournal of Molecular Biology, 1970
- Translation of RNA from Encephalomyocarditis Virus in a Mammalian Cell-free SystemNature, 1970