Simultaneous measurement of thyroxine and triiodothyronine in trout plasma using a solid-phase radioimmunoassay
- 1 August 1984
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 62 (8) , 1451-1453
- https://doi.org/10.1139/z84-208
Abstract
A solid-phase radioimmunoassay (RIA) employing miniature G-25 Sephadex columns and a single isotope was evaluated for simultaneous measurement of T3 (3,5,3''-triiodo-L-thyronine) and T4 (L-thyroxine) in trout plasma. The method was judged reliable based on the characteristics of the standard curves, the high and consistent recoveries of T3 (87.0 or 86.7%) and T4 (103 or 98%) added either singly or in combination, low inter- (< 8%) and intra-assay (< 7%) coefficients of variation, predictable values of T3 and T4 after plasma dilution, and acceptable correlations between hormone values obtained using either the simultaneous or single RIA methods (rT3 = 0.89; rT4 = 0.91). The simultaneous RIA with its savings in time, plasma volume, and reagents can be used to advantage to measure T3 and T4 in plasma of trout and presumably other vertebrates.Keywords
This publication has 1 reference indexed in Scilit:
- Measurement of L-thyroxine and 3,5,3′-triiodo-L-thyronine levels in fish plasma by radioimmunoassayCanadian Journal of Zoology, 1977