Intein-mediated in vitro synthesis of lipidated Ras proteins
- 28 November 2005
- journal article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 3,p. 260-262
- https://doi.org/10.1039/b511736d
Abstract
Fully functional lipid-modified Ras proteins suitable for the study of Ras–membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.Keywords
This publication has 14 references indexed in Scilit:
- Synthesis of Functionalized Rab GTPases by a Combination of Solution‐ or Solid‐Phase Lipopeptide Synthesis with Expressed Protein LigationChemistry – A European Journal, 2005
- An Acylation Cycle Regulates Localization and Activity of Palmitoylated Ras IsoformsScience, 2005
- Chemical biology of protein lipidation: semi-synthesis and structure elucidation of prenylated RabGTPasesOrganic & Biomolecular Chemistry, 2005
- Synthesis and Application of Fluorescent Ras Proteins for Live‐Cell ImagingChemBioChem, 2005
- Synthesis of Fluorescently Labeled Mono- and Diprenylated Rab7 GTPaseJournal of the American Chemical Society, 2004
- Semisynthesis of Proteins by Expressed Protein LigationAnnual Review of Biochemistry, 2003
- Ras—A Molecular Switch Involved in Tumor FormationPublished by Wiley ,2000
- Synthesis of Native Proteins by Chemical LigationAnnual Review of Biochemistry, 2000
- The farnesyl group activates Ras toward guanine nucleotide exchange catalyzed by the SOS proteinBioorganic & Medicinal Chemistry Letters, 1997
- Protein Splicing Involving the Saccharomyces cerevisiae VMA InteinJournal of Biological Chemistry, 1996