Instrument To Detect Near-Infrared Fluorescence in Solid-Phase Immunoassay
- 1 October 1994
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 66 (19) , 3102-3107
- https://doi.org/10.1021/ac00091a018
Abstract
The construction of a near-infrared (near-IR) fluorescence detector for measuring picomolar levels of near-IR laser dyes is described. The detector is designed for use in an immunoassay technique that employs antibodies labeled with near-IR polymethine cyanine dyes. These dyes possess spectral properties that are exclusive to the near-IR region (650-1100 nm). The instrumentation is characterized, including its hardware and data acquisition software components. The detector is capable of measuring fluorescence in both solution and solid-phase environments. Data on the detector's performance is presented.Keywords
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