Single-Cell Enumeration of an Uncultivated TM7 Subgroup in the Human Subgingival Crevice
- 1 October 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (10) , 6294-6298
- https://doi.org/10.1128/aem.69.10.6294-6298.2003
Abstract
Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.Keywords
This publication has 15 references indexed in Scilit:
- Prevalence of Bacteria of Division TM7 in Human Subgingival Plaque and Their Association with DiseaseApplied and Environmental Microbiology, 2003
- Fluorescence in situ hybridization of 16S rRNA gene clones (Clone‐FISH) for probe validation and screening of clone librariesEnvironmental Microbiology, 2002
- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ HybridizationPublished by Springer Nature ,2001
- Bacterial Diversity in Human Subgingival PlaqueJournal of Bacteriology, 2001
- The RDP-II (Ribosomal Database Project)Nucleic Acids Research, 2001
- Investigation of Candidate Division TM7, a Recently Recognized Major Lineage of the Domain Bacteria with No Known Pure-Culture RepresentativesApplied and Environmental Microbiology, 2001
- DNA Segregation in BacteriaAnnual Review of Microbiology, 2000
- Mechanism of DNA segregation in prokaryotes: ParM partitioning protein of plasmid R1 co-localizes with its replicon during the cell cycleThe EMBO Journal, 1999
- Phylogenetic Oligodeoxynucleotide Probes for the Major Subclasses of Proteobacteria: Problems and SolutionsSystematic and Applied Microbiology, 1992
- Synthetic oligonucleotide probes deduced from amino acid sequence dataJournal of Molecular Biology, 1985