Nucleotide Sequence (n= 159) of the Amino‐Acid‐Accepting 3′‐OH Extremity of Turnip‐Yellow‐Mosaic‐Virus RNA and the Last Portion of Its Coat‐Protein Cistron

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Abstract
The sequence of the 3''-OH terminal 159 nucleotides of turnip yellow mosaic virus RNA was established. Uniformly 32P-labeled turnip yellow mosaic virus RNA was partially digested with T1 RNase and the fragments were fractionated by polyacrylamide gel electrophoresis. Fragments originating from the 3''-OH end of the RNA molecule were identified by testing for the 3''-terminal oligonucleotide, C-COH, after total U2 RNase hydrolysis. Once identified, the 3''-OH terminal fragments were sequenced. The first 51 nucleotides of the longest of the sequenced fragments (158 nucleotides) extend into the 3''-terminal part of the coat protein cistron. The coat protein cistron is followed by a stretch of 108 untranslated nucleotides whose function, though still unknown, is probably linked to the tRNA-like properties which were attributed to the 3''-OH extremity of this viral RNA. Two possible secondary structures are proposed for the sequence, and the implications of the findings with regard to the tRNA-like properties of the extremity are discussed.