Microbiological Safety Evaluation of a Solid and Liquid Pathological Incinerator

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Abstract
A semiportable oil or gas-fired incinerator was designed, fabricated and installed for use at a primate research laboratory. The incinerator was challenged with Bacillus subtilis var. niger spores to determine minimum operating temperatures to prevent the release of spores to the atmosphere when contained in either solid or liquid refuse. The theoretical spore challenge concentration when mixed with the incinerator combustion air for the solid refuse was 3 x 10^7 spores/ft^3 (8.4 x 10^5 spores/m^3), and 7.2 x 10^2 spores/ ft3 (20 spores/m^3) for the liquid refuse. To prevent the release of viable microorganisms to atmosphere the maintained sterilization temperatures for this incinerator were determined to be 760 °C (1,400°F) in the primary chamber and 871 °C (1,600 °F) in the secondary chamber.

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