Z Form of Poly d(A-T) · Poly d(A-T) in Solution Studied by CD and UV Spectroscopies

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Abstract
Near UV CD spectra, UV absorption spectra and their first derivatives have been recorded on poly d(A-T) · poly d(A-T) solutions in presence of high NaCl concentration and various amounts of NiCl2. Comparison of the results presented here with those obtained for poly d(G-C) · poly d(G-C) and poly d(A-C) · poly d(G-T) in comparable conditions, and the I.R. and Raman data on poly d(A-T) · poly d(A-T), allows us to assign the new spectra to the Z conformation of poly d(A-T) · poly d(A-T) in solution. The mechanism by which nickel ions induce the B→Z interconversion in the presence of high NaCl concentration is discussed.