STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIORIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE

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Abstract
Cells cultured in a desthiobiotin-limiting and S-deficient medium showed a marked ability for biotin formation from desthiobiotin and various S sources. The most effective S compounds were methionine sulfoxide and methionine, followed by sodium sulfide, sodium sulfite, sodium sulfate, homocysteine, S-adenosylmethionine, and methylmercaptan. Cysteine, cystine, methionine sulfone, 5[image]-methyladenosine, choline sulfate, and glutathione were less effective. In the case of sodium sulfate, further addition of glucose in combination with desthiobiotin was necessary after pre-incubation, but not necessary in the case of methionine. Biotin formation by biotin-deficient cell suspension was inhibited by ethionine and was restored only by addition of methionine.

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