Motility and Morphology of Human Spermatozoa in Split Ejaculates

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Abstract
Sperm concentration and 4 motility characteristics (percentage of motile spermatozoa, average velocity, average straight line approach and average speed constancy) were determined in 3 fractions of human split ejaculates from healthy volunteers. A semi-automatic analysis technique was used to determine sperm parameters from multiple exposure photographs (MEP). Sperm concentration and percentage motility were significantly higher in the 1st fraction as compared to the 3rd fraction of split ejaculates. The average spermatozoal velocity, average speed constancy and average straight line approach were the same in all fractions. Differences in the percentage of motile spermatozoa were also found after collection of split ejaculates in culture medium. This phenomenon may be caused by differences in sperm morphology. Significantly more normal spermatozoa were present in the 1st fractions of 19 split ejaculates as compared to the last fractions.