Biosynthesis of streptomycin enzymatic formation of dihydrostreptomycin 6-phosphate from dihydrostreptosyl streptidine 6-phosphate.

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Abstract
Incubation of O-.alpha.-L-dihydrostreptose (1 .fwdarw. 4) streptidine 6-phosphate (I) with a protein-free extract from Streptomyces griseus as endogenous donor and a cell-free extract from this organism led to formation of dihydrostreptomycin 6-phosphate (II). The product was identified by paper chromatography and by its degradation to dihydrostreptobiosamine (III). II was not formed when either the donor solution or the dialysed cell-free extract was omitted. The results corroborate the role of I as intermediate in streptomycin biosynthesis. The synthesis of I from dTDP-L-dihydrostreptose, streptidine 6-phosphate and a dihydrostreptosyltransferase from S. griseus was shown previously.