Leukocyte Culture and Chromosome Preparations from Adult Dog Blood
- 1 January 1965
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 40 (6) , 317-320
- https://doi.org/10.3109/10520296509116437
Abstract
Plasma is obtained from dog blood after 3 hr settling in a syringe. Portions of the plasma (0.5-1.0 ml) are added to 4 ml of a medium consisting of 17 parts of BME Spinner, 3 parts of calf serum, 0.5 parts of glutamine, 0.5 parts of penicillin-streptomycin, and 0.1-1.0 parts of Scarlet Runner bean phytohemagglutinin. Colchicine, 0.1 ml of 10:1 stock solution, is added after 72 hr and incubation continued for 2 hr, then centrifuged 5 min at 700 rev/min. The supernatant is discarded, 3 ml of distilled water added, and the cell suspension centrifuged again. The supernatant is discarded and the fixative, consisting of 45% glacial acetic acid allowed to act for 0.5 hr. Acetic-orcein stains of smears were very satisfactory.This publication has 4 references indexed in Scilit:
- Culture and Slide Preparation of Leukocytes from Blood ofIMACACAStain Technology, 1964
- Chromosome preparations of leukocytes cultured from human peripheral bloodExperimental Cell Research, 1960
- An Air-Drying Technique for Flattening Chromosomes in Mammalian Cells GrownIn VitroStain Technology, 1958
- MAMMALIAN CHROMOSOMES IN VITROJournal of Heredity, 1953