Fluorescent, Through-Bond Energy Transfer Cassettes for Labeling Multiple Biological Molecules in One Experiment
- 11 November 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (48) , 14668-14669
- https://doi.org/10.1021/ja037193l
Abstract
Through-bond energy transfer cassettes based on a fluorescein donor component electronically conjugated to rhodamine-like acceptors have been designed and synthesized. They absorb strongly at 488 nm (Ar-laser emission) and efficiently transfer the energy to the acceptor component that emits strongly. Further, the cassettes are more stable to photobleaching than fluorescein, making them potentially more suitable for single-molecule detection methods than fluorescein itself. These studies form the basis for improved detection of chain-terminated DNA in high-throughput sequencing and other applications in biotechnology.Keywords
This publication has 5 references indexed in Scilit:
- Fluorescence resonance energy transfer terminators for DNA sequencingTetrahedron Letters, 2000
- Principles of Fluorescence SpectroscopyPublished by Springer Nature ,1999
- Photophysics and Mechanisms of Intramolecular Electronic Energy Transfer in Bichromophoric Molecular Systems: Solution and Supersonic Jet StudiesChemical Reviews, 1996
- Coordinated Exosat and spectroscopic observations of flare stars and coronal heatingNature, 1986
- A convenient synthesis of acetylenes: catalytic substitutions of acetylenic hydrogen with bromoalkenes, iodoarenes and bromopyridinesTetrahedron Letters, 1975