Xyloglucan and rβ-D-Glucan in Cell Walls of Rice Seedlings

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Abstract
Cell walls of 4-day old rice seedlings were extracted successively with ammonium oxalate-oxalic acid, 4% KOH and 24% KOH. A rβ-D-glucan preparation and a xyloglucan preparation were isolated from the 4% KOH extract and 24% KOH extract, respectively. Methylation analysis and enzymic degradation studies of the polysaccharides showed that the former was built up predominantly of repeating-oligosaccharide units of 3-O-rβ-cellobiosyl-D-glucose and 3-O-rβ-cellotriosyl-D-glucose in a molar ratio of 2.6 : 1.0, and the latter was of repeating-oligosaccharide units of α-D-xylosyl-(1→6)-rβ-D-glucosyl-(1→4)-[α-D-xylosyl-(1→6)]-rβ-D-glucosyl-(1→4)-D-glucose, α-D-xylosyl-(1→6)-rβ-D-glucosyl-(1→4)-D-glucose and cellobiose.