Enhanced nisin production by increasing genes involved in nisin Z biosynthesis in Lactococcus lactis subsp. lactis A164
- 1 February 2005
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 27 (3) , 155-160
- https://doi.org/10.1007/s10529-004-7661-3
Abstract
Nisin Z production in Lactococcus lactis subsp. lactis A164 was improved by introducing multicopy genes, nisZ, nisRK, or nisFEG, involved in nisin biosynthesis into A164 strain. A similar growth profile was obtained from all strains tested. However, the cells expressing nisRK produced 25,000 AU nisin Z ml−1 compared to 16,000 AU ml−1 by the control strain. Northern blot analysis revealed that over-expression of nisRK promoted the transcription of the nisZ gene. The A164 strain expressing multicopy nisFEG also had an increased nisin Z production (25,000 AU ml −1) but produced the nisin more slowly than the cells expressing multicopy nisRK.Keywords
This publication has 13 references indexed in Scilit:
- Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatographyBiotechnology Letters, 2004
- Function of Lactococcus lactis Nisin Immunity Genes nisI and nisFEG after Coordinated Expression in the Surrogate Host Bacillus subtilisJournal of Biological Chemistry, 2003
- Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from kimchiJournal of Biotechnology, 2002
- Expression of Staphylococcus aureus Clumping Factor A in Lactococcus lactis subsp. cremoris Using a New Shuttle VectorInfection and Immunity, 2000
- Improving nisin production by increasing nisin immunity/resistance genes in the producer organism Lactococcus lactisApplied Microbiology and Biotechnology, 1998
- Functional analysis of promoters in the nisin gene cluster of Lactococcus lactisJournal of Bacteriology, 1996
- Autoregulation of Nisin Biosynthesis in Lactococcus lactis by Signal TransductionJournal of Biological Chemistry, 1995
- Bacteriocins of Lactic Acid BacteriaPublished by Springer Nature ,1994
- Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB proteinApplied and Environmental Microbiology, 1992
- Transfer of sucrose fermenting ability, nisin resistance and nisin production intoStreptococcus lactis712FEMS Microbiology Letters, 1984