Studies on carbohydrate-metabolizing enzymes. 2. Trans-α-glucosylation by extracts of Tetrahymena pyriformis

Publisher Website
Google Scholar
Abstract
Cell-free extracts of the ciliate Tetrahymenapyriformis show hydrolytic activity towards maltose, isomaltose, cellobiose, sucrose, methyl [alpha]-D-glucoside, [alpha]-D-glucosyl phosphate and starch. In concentrated solution, oligosaccharides are synthesized from maltose and from isomaltose, but not from glucose. Oligosaccharide synthesis from maltose results in the formation of isomaltose, malto-triose, panose, 63-[alpha]-glucosylmaltotriose and maltotetraose. The acceptor specificity for [alpha]-glucosyl radicals by the protozoal enzyme system and other maltases is discussed.