Multilayer immobilized‐enzyme filter reactors: Urease bound to nylon fabric filters

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Abstract
Urease was bound to commercially available nonwoven nylon fabric filters. Multilyer immoibilized‐enzyme filter reactors were constructed by packing varying numbers of urease‐nylon filters in a column. Owing to the relatively open structure and high mechanical strength of the filter fabric, compaction and pressure drop effects were minimal. The reactors could be operated in a wide range of substrate concentrations and flow rates under conditions where mass‐transfer limitations could be neglected. The kinetic behavior of the immobilized‐enzyme filter reactors could be described by a linear form of the integrated Michaelis‐Menten equation using a model based on the sequential action of the enzyme filters.