Purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli
- 1 July 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 213 (1) , 187-191
- https://doi.org/10.1042/bj2130187
Abstract
A procedure for the purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli is described. Homogeneous enzyme of specific activity 17.7 units/mg was obtained in 22% yield. The key purification step involves substrate elution of the enzyme from a cellulose phosphate column. The subunit Mr was estimated to be 49 000 by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The native Mr was estimated to be 55 000 by gel filtration, indicating that the enzyme is monomeric.This publication has 30 references indexed in Scilit:
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