Fragmentation of the Nucleus in Rous Sarcoma Virus-Infected Chick Embryo Cells. II. Structural and Metabolic Studies2

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Abstract
Structural and metabolic studies were designed to obtain additional information as to the nature of the nuclear fragmentation process in Rous sarcoma virus-infected chick embryo cells. No obvious alterations in the fragmented nuclei could be seen in electron micrographs, and the amount of DNA in the fragmented nuclei was similar to that in normal-shaped nuclei. The maximum percentage of cells with fragmented nuclei was approximately 10%, even with a multiplicity of infection of 1. Infected cultures passaged 7 times contained 10% of cells with fragmented nuclei, which indicated either survival or continued formation of these cells. Autoradiographic studies demonstrated that thymidine, uridine, and leucine incorporation was the same or greater in cells with fragmented nuclei as in cells with normal nuclei. Exposure of infected cells to bromodeoxyuridine enhanced the formation of fragmented nuclei.

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