Some Properties of a tobacco Ringspot Virus Isolate from South Australia

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Abstract
A virus has been isolated from Gladiolus sp. (cv. Spic and Span) in South Australia. This gladiolus virus has been partially purified and could not be distinguished serologically from a North American strain of tobacco ringspot virus although it is symptomologically unlike the type strain. Gladiolus virus was not transmitted by the aphids Myzus persicad (Sulz.), Aphis craccivora (Koch), Aphis gossypii (Glov.), Hyperomyzus lactucae (L.), or Macrosiphum euphorbiae (Thos. ). The virus failed to be transmitted through soil infested with the nematode Xiphinema americanum Cobb, but was transmissible through seeds of Glycine max Merr. (cv. Lincoln) and Nicotiana glutlnosa L. Partially purified preparations of gladiolus virus contained poly-hedral particles about 29 m[mu] in diameter and in the analytical ultra-centrifuge showed four peaks of 21, 57, 99, and 136S. The 21 and 57S components were proteins whereas the 99 and 136S components were nucleoproteins containing about 21 and 35% ribonucleic acid respectively. The molar base ratios guanine: adenine: cytosine: uracil of gladiolus virus are 23-8: 22-3: 22-5: 31-4.