Destruction of photosystem I iron‐sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures
Open Access
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2) , 235-238
- https://doi.org/10.1016/0014-5793(95)00254-7
Abstract
The activity of photosystem (PS) I in cucumber leaves was selectively inhibited by weak illumination at chilling temperatures with almost no loss of P‐700 content and PSII activity. The sites of inactivation in the reducing side of PSI were determined by EPR and flash photolysis. Measurement by EPR showed the destruction of iron‐sulfur centers, FX, FA and FB,in parallel with the loss of quantum yield of electron transfer from diaminodurene to NADP+. Flash photolysis showed the increases in the triplet states of P‐700 and antenna pigments, along with the decrease in the electron transfer from P‐700 to FA/FB. This indicates the increase in the charge recombination between P‐700+ and A0 −. It is concluded that weak‐light treatment of cucumber leaves at chilling temperature destroys FX, FA and FB and possibly A1. This gives the molecular basis for the mechanism of selective PSI photodamage that was recently reported [Sonoike and Terashima (1994) Planta 194, 287–293].Keywords
This publication has 16 references indexed in Scilit:
- Photoinhibition of Photosystem II. Inactivation, protein damage and turnoverPublished by Elsevier ,2003
- The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem IIPlanta, 1994
- Mechanism of photosystem-I photoinhibition in leaves ofCucumis sativus L.Planta, 1994
- Selective destruction of iron-sulfur centers by heat/ethylene glycol treatment and isolation of Photosystem I core complexBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopyBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- Identification of electron-transfer reactions involving the acceptor A1 of photosystem I at room temperatureBiochemistry, 1989
- Picosecond absorbance changes upon selective excitation of the primary electron donor P-700 in Photosystem IBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1986
- Photoinhibition of Photosynthesis Induced by Visible LightAnnual Review of Plant Physiology, 1984
- Photoinhibition of Reaction Centers of Photosystems I and II in Intact Bryopsis Chloroplasts under Anaerobic ConditionsPlant Physiology, 1982
- A further study of P430: A possible primary electron acceptor of photosystem IArchives of Biochemistry and Biophysics, 1971