On the 48÷80-induced secretion of tissue mast cells and its mitogenic effect on nearby cells in the intact rat

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Abstract
Histamine release from tissue-bound mast cells and cell proliferation in the proper mesentery in the intact rat was quantitated following an intraperitoneal injection of graded doses of compound 48÷80. The doseresponse curves were sigmoid-like in linear-log plots. ED50 for histamine release was 0.035–0.040 and for increased cell proliferation 0.040–0.048 μg per g BW. The proliferative response following mast-cell secretion ceased after a period of between 48–72 h, irrespective of whether a high or a low dose of 48÷80 was used. Based on the net rate of histamine synthesis (ca. 0.45 μg/g mesentery wet weight/h) after an initial injection of 48÷80, on the extent of histamine release and the proliferative response after a repeated injection of 48÷80, it is concluded that there is a lag period of at least 3 days before proliferation can be re-stimulated by renewed 48÷80-induced mast-cell secretion.