Glutaryl Acylases: One‐Reaction Enzymes or Versatile Enantioselective Biocatalysts?
- 3 June 2003
- journal article
- research article
- Published by Wiley in Advanced Synthesis & Catalysis
- Vol. 345 (6-7) , 783-789
- https://doi.org/10.1002/adsc.200303013
Abstract
A significant broad substrate specificity, that crosses over the usual β‐lactam derivatives, has been observed with an industrial glutaryl‐7‐aminocephalosporanic acid acylase (GA). This enzyme possesses significant enantioselective amidase and even esterase activity, with a stereopreference for the S‐enantiomer. The easy separation of products from unreacted reagents, possessing different physical‐chemical properties, is achieved by solvent extraction, avoiding chromatography or distillation during reaction work‐up.Keywords
This publication has 20 references indexed in Scilit:
- Kinetic resolutions of racemic amines and alcohols catalyzed by an industrial glutaryl-7-aminocephalosporanic acid acylase with unexpected broad substrate specificityTetrahedron: Asymmetry, 2003
- Structure‐based prediction of modifications in glutarylamidase to allow single‐step enzymatic production of 7‐aminocephalosporanic acid from cephalosporin CProtein Science, 2002
- Highly efficient and enantioselective enzymatic acylation of amines in aqueous mediumTetrahedron: Asymmetry, 2001
- The Role of α-Amino Group of the N-terminal Serine of β Subunit for Enzyme Catalysis and Autoproteolytic Activation of Glutaryl 7-Aminocephalosporanic Acid AcylasePublished by Elsevier ,2000
- The 2.0 Å Crystal Structure of Cephalosporin AcylaseStructure, 2000
- A protein catalytic framework with an N-terminal nucleophile is capable of self-activationNature, 1995
- High‐level Production, Chemical Modification and Site‐directed Mutagenesis of a Cephalosporin C Acylase from Pseudomonas Strain N176European Journal of Biochemistry, 1995
- Quantitative analyses of biochemical kinetic resolutions of enantiomersJournal of the American Chemical Society, 1982
- Isolation and properties of 7.BETA.-(4-carboxybutanamido)cephalosporanic acid acylase-producing bacteria.Agricultural and Biological Chemistry, 1981
- Chemistry of cephalosporin antibiotics. XIV. Reaction of cephalosporin C with nitrosyl ChlorideJournal of the American Chemical Society, 1969